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. 2008 May 6;13(5-2):454–463. doi: 10.1016/j.ccr.2008.03.004

Figure 1.

Figure 1

Effect of Tenovin-1 on Cultured Tumor Cell Lines

(A) Tenovin-1 structure.

(B) MCF-7 cells (expressing wild-type p53) were treated with 10 μM tenovin-1 for the indicated times. p53 levels and p21 levels were detected using DO1 (Bartkova et al., 1993) and 118 (Fredersdorf et al., 1996) mouse monoclonal antibodies, respectively. An antibody against α-tubulin (Cat. No. T9026, Sigma) was used to monitor loading efficiency.

(C) MCF-7 cells were treated with 10 μM tenovin-1 for the indicated times, and p53 and p21 mRNA levels were analyzed by Taqman-PCR as described (Saville et al., 2004). Error bars correspond to standard deviation values (n = 3).

(D) Toxicity of tenovin-1 on cultured tumor cells. Tumor cell lines were treated with DMSO (control) or with 10 μM tenovin-1 for 48 hr. Cell death (necrosis and apoptosis) was measured by annexin-V/propidium iodide labeling and FACS. Values correspond to the average of two independent experiments (±SD). p53 status in each cell line is indicated (DN, coexpressing the dominant-negative form of p53).