Figure 4.

Yeast Genetic Screen to Identify Tenovin-6 Hypersensitive Yeast Strains from within a Genome-wide Heterozygous Gene Deletion Collection

(A) Plot of % control growth at 16.7 μM tenovin-6 versus growth in the absence of compound (OD₆₀₀) for 6261 heterozygous gene deletion strains showing the positive correlation between growth in the presence of compound and overall growth in its absence. Outlier strains (511) showing potential hypersensitivity were identified as shown.

(B) Examples of data from a secondary screen in which growth of a range of initial cell concentrations plus and minus compound were plotted. YDL010W is an example of a nonhypersensitive strain while YDL041W is the hypersensitive strain heterozygous for a SIR2 truncation. Graphs show the coefficient of linear correlation (R²) and best-fit equations giving the slope parameter used to generate the hypersensitivity index.

(C) Histogram showing the distribution of hypersensitivity index values for 408 strains giving reproducible data and indicating the position of the YDL041W heterozygote (SIR2).