. 2008 May 6;13(5-2):454–463. doi: 10.1016/j.ccr.2008.03.004

Table 1.

Tenovin SAR Studies Aimed at Increasing Water Solubility

ID			p53 Increase^a	K40Ac Tubulin Increase^b	SirT2 Inhibition^c
ID	R¹	R²	p53 Increase^a	K40Ac Tubulin Increase^b	SirT2 Inhibition^c
Tenovin-1	^tBu	NHCOCH₃	+	+	+
Tenovin-2	^tBu	NHCOCH(CH₃)₂	+	+	+
Tenovin-3	^tBu	NH₂	+	+	+
Tenovin-4	N₃	NHCOCH₃	−	−	−
Tenovin-5	^tBu	NHCOPh	+	+	+
Tenovin-6	^tBu	NHCO(CH₂)₄NMe₂.HCl	+	+	+

Ability to increase p53 levels was determined in MCF-7 cells treated for 6 hr at 10 μM as described in Figure 1B.

Ability to increase K40 Ac-tubulin levels was determined in H1299 cells treated as described in Figure 7B.

SirT2 inhibition assessed as described in Figure 5B using a compound concentration of 10 or 30 μM depending on the compound's solubility in the assay buffer.