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. 2009 Oct 2;5(10):e1000669. doi: 10.1371/journal.pgen.1000669

Figure 2. Extended transition zone chromosome morphology and lack of chiasmata in meiotic prophase I nuclei in syp-4 mutants.

Figure 2

(A) Schematic representation of the predicted SYP-4 protein. The region deleted in the tm2713 mutant allele is indicated (codons 28 through 84). Coiled-coil domains, with start and end points identified through the COILS program are shaded in dark gray. The black bar indicates the N-terminal region used for antibody production. (B) Low magnification images of DAPI-stained nuclei of whole-mount gonads from age-matched wild type and syp-4(tm2713) adult hermaphrodites. The extended transition zone chromosome configuration is observed until late pachytene in the latter. Progression from early to mid-prophase is observed from left to right. (C–H) High magnification images of DAPI-stained nuclei at transition zone (C,F), pachytene (D,G), and diakinesis (E,G) in wild type (C–E) and syp-4(tm2713) mutants (F–H). Bars, 5 µm (B) and 2 µm (C–H).