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. 2009 Oct 2;5(10):e1000669. doi: 10.1371/journal.pgen.1000669

Figure 3. SYP-4 localizes to chromosomes during prophase I, and its localization requires both axis-associated and CR proteins.

Figure 3

(A–C) High magnification images of nuclei stained with DAPI and anti-SYP-4. Wild type nuclei at transition zone (A), late pachytene (B), and diakinesis (C), showing immunolocalization of SYP-4 on chromosomes throughout meiotic prophase I. Arrow in (A) indicates a premeiotic nucleus adjacent to the meiotic transition zone nuclei. Arrow in (C) indicates two superimposed bivalents. Inset in (C) indicates a single bivalent at high magnification, co-immunostained with SYP-4 (red) and HTP-3 (green). SYP-4 localizes to the region distal to the chiasma (short arms of the bivalent). (D) Pachytene nuclei exemplifying the lack of SYP-4 immunolocalization on chromosomes observed in syp-4 mutants. (E–G) SYP-4 is excluded only from unsynapsed chromosomes (arrows) observed in him-8 mutants. (H–K) SYP-4 localization in pachytene nuclei is perturbed in him-3 (H), syp-1 (I), syp-2 (J), and syp-3 (K) mutants, indicating that SYP-4 localization requires both normal axis-morphogenesis and the presence of central region proteins. Bars, 2 µm.