Figure 4. Time-dependent stimulation of EGFP-LC3 processing and inhibition of mTORC1 signaling by perhexiline, niclosamide, amiodarone and rottlerin.

MCF-7 cells stably expressing EGFP-LC3 were treated for the indicated times with 10 µM perhexiline, 10 µM niclosamide, 50 µM amiodarone or 3 µM rottlerin. Immunoblotting was carried out using the antisera indicated.