Fig. 2.

Oxidative stress can be induced in NSC34 cells by serum withdrawal. (a) DCF fluorescence in wild-type NSC34 cells under basal conditions and (b) after serum withdrawal. Scale bar, 50 μm. (c) Serum withdrawal induced a threefold increase in DCF fluorescence in wild-type NSC34 cells. Graph shows means ± 1 SEM. (d) Ebselen reduced serum withdrawal-induced oxidative stress, measured by DCF fluorescence, in NSC34 cells in a dose-dependent manner, with a half-maximal effect (EC₅₀) of 4 μM. There was no increase in toxicity, as measured by EthD1 fluorescence.