Figure 6.

RSV infection down-regulates Nrf2 expression. A549 cells were infected with RSV for various lengths of time and harvested to prepare nuclear extracts. (A) Nuclear amounts of Nrf2 protein were determined by Western blot. Nrf2 gene expression after RSV infection was quantified in (B) A549 cells or (C) SAE cells by real-time PCR. Open bars, control; solid bars, RSV. Data are presented as fold changes and are representative of two independent experiments. *P < 0.05 relative to uninfected cells.