Figure 3.

The CoA-IT inhibitor, Et-18-O-CH3, induced eosinophil apoptosis in vitro. (A) Human eosinophils were cultured for 0 to 72 hours in media alone (control, solid bars), or with Et-18-O-CH3 10 μM (hatched bars) or 25 μM (open bars). Apoptosis was determined as the percent of eosinophils with decreased cell volume as shown by Coulter counter measurements. Samples were taken at 0, 24, 48, or 72 hours. (B). Eosinophils were cultured for 0 to 72 hours in media with IL-5 alone (50 pg/ml, solid bars), or combination with Et-18-O-CH3 at 10 μM (hatched bars) or 25 μM (open bars). Apoptosis was determined as the percent of eosinophils with decreased cell volume as shown by Coulter counter measurements. Samples were taken at 0, 24, 48, or 72 hours. Significant differences of P < 0.05 were seen between eosinophils treated with IL-5 and Et-18-O-CH3 + IL-5 after 48 hours, as indicated by asterisks (n = 3–4 separate cell preparations per assay).