Figure 3. Loss of functions associated with the Runx2 C-terminus prevents cell growth and tumor cell like phenotype.
(A) Expression levels of ectopic wild type or mutant Runx2 proteins in MDA-MB-231 cells (ΔC: C-terminal deletion; double point mutant RY: Y428A &R398A) by lentiviral mediated gene delivery is shown by western blot analysis. (B) MDA-MB-231 cells stably expressing Runx2 mutants show significantly smaller acini in mutant cell lines compared to empty vector control. Cells expressing wild type Runx2 formed large and disorganized structures compared to empty vector control cells.
