Fig. 13.

Influence of temperature on the action of 2-APB and ryanodine. Representative responses showing the change in airway area with respect to time on exposure to 200 nM MCh and subsequently 50 and 100 μM 2-APB (A) or 1 μM (light gray line), 10 μM (gray line), or 50 μM (black line) ryanodine (C) at 37°C are shown. Summaries of the relaxation induced in airways contracted with 200 nM MCh by 50 and 100 μM 2-APB (B) or 1, 10, or 50 μM ryanodine (D) at 37°C. The extent of relaxation (percent) was measured after 10 min of 2-APB or ryanodine exposure. Each column represents the mean ± SE from ≥5 different experiments from 3 mice.