Fig. 14.

Influence of temperature on MCh-induced Ca²⁺ signaling. Representative responses showing the intracellular Ca²⁺ signaling of airway SMCs in response to 200 nM MCh (A) and subsequently 100 μM 2-APB (B) or 50 μM ryanodine (C) at 37°C are shown. MCh induced rapid (∼60 min⁻¹) and sustained Ca²⁺ oscillations. After the MCh-induced Ca²⁺ oscillations had stabilized (∼3 min), 2-APB or ryanodine was added to the lung slice. The Ca²⁺ oscillations were inhibited by 2-APB (B) but unaffected by ryanodine (C) after 3 min. Representative data are from ≥6 slices from 3 mice for each antagonist. In contrast, Ca²⁺ oscillations induced by KCl (50 mM) were rapidly stopped by 50 μM ryanodine (D), and this was associated with an increase in baseline Ca²⁺. Representative data are from ≥5 different airways from 3 mice.