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. 2009 May 22;297(2):L238–L250. doi: 10.1152/ajplung.90591.2008

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Fig. 1. — Hypoxia induces a progressive accumulation of leukocytic cells expressing CD11b in the rat pulmonary artery (PA) adventitia. Cryosections of PAs from normoxic (A), 7-day hypoxic (B), and 28-day hypoxic (C) rats were stained with antibodies against CD11b (red) and cell nuclei [4′,6′-diamidino-2-phenylindole (DAPI), blue]. Scale bar is 100 μm; all pictures were taken at ×10 magnification. D: quantification of the number of leukocytic cells expressing CD11b (CD11b+ cell index) in the rat PA over time. One-day hypoxic rats show no difference in the number of CD11b+ cells compared with controls, whereas 7- and 28-day hypoxic show a progressive increase in the number of CD11b+ cells. In addition, leukocytic cells are found further away from the external elastic lamina in the 7- and 28-day hypoxic rats due to the increased thickness of the adventitia.