Cryopyrin and ASC are required for zymosan- and mannan-induced caspase-7 activation. BMDMs were left untreated (CTRL) or stimulated with zymosan, mannan, or LPS for 4 h, the last 30 min in the presence of PBS (zymosan, mannan) or 5 mm ATP (zymosan+ATP, mannan+ATP, LPS+ATP). Cell extracts were immunoblotted for caspase-7. Black arrowheads indicate full-length caspase-7 (33 kDa), and white arrowheads mark the large subunits of activated caspase-7 (19 kDa). Data shown are representative of three separate experiments.