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. 2009 Jun 4;284(31):20593–20601. doi: 10.1074/jbc.M109.021477

FIGURE 4.

FIGURE 4.

DLX5 promotes Jurkat cell proliferation by regulating the expression of MYC. A, expression of MYC protein in Jurkat cells transfected by vector (1) or DLX5 (2). The histogram in A represents the density of the MYC bands in immunoblot. B, real time RT-PCR analysis of MYC mRNA levels in Jurkat cells transfected by vector and DLX5. C, expression of MYC in Jurkat cells transfected with increasing amounts of DLX5 plasmid. D, real time RT-PCR analysis of MYC mRNA level in Jurkat cells transfected with increasing amounts of DLX5 plasmid. E, cell numbers counted at 0, 24, and 48 h in Jurkat cells transfected with vector (Vec), DLX5, DLX5 + non-targeting siRNA (siCon), DLX5 + siRNA1 against MYC (siMYC1), DLX5 + siRNA2 against MYC (siMYC2), and MYC. F, expression of MYC, DLX5, and β-ACTIN in Jurkat cells transfected with Vec (1), DLX5 (2), DLX5 + non-targeting siRNA (3); DLX5 + siRNA1 against MYC (4), DLX5 + siRNA2 against MYC (5), and MYC (6).