FIGURE 3.

FPLC gel filtration of CK2 subunits and holoenzymes. Individual recombinant A. thaliana CK2 subunits (300 μg), CK2 holoenzymes (300 μg), and a series of standards (300 μg) were applied to an FPLC HiPrep 16/60 Sephacryl S-200 HR column (126-ml bed volume; GE Healthcare) or FPLC HiLoad 16/60 Superdex S200 column (126-ml bed volume; GE Healthcare) in Buffer B-180, and the elution was monitored by A₂₈₀ for 120 ml. Holoenzymes were formed by incubating CK2 subunits at a 1:1 molar ratio on ice for 1 h. A, representative elution pattern for individual subunits CK2α1, CKβ1, and holoenzyme CK2α1β1 on the HiPrep 16/60 Sephacryl S-200 HR column is shown. B, summary of elution volumes from gel filtration.