FIGURE 5.

Caspase-8 and -9 activation by menadione with different ways of emptying calcium stores in pancreatic acinar cells is shown. Caspase-8 (A) and -9 (B) activation by menadione when calcium content of ER was reduced slowly (30 nm thapsigargin followed by 10 μm thapsigargin) or quickly (10 μm thapsigargin) or with 100 nm Bafilomycin A1. Bars represent percent of apoptotic cells. C shows general caspase substrate controls in the presence of thapsigargin and Bafilomycin A1. D shows that caspase-9 activation is significantly inhibited by the mitochondrial uniporter inhibitor Ru360.