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. 2009 Jun 9;284(31):20812–20821. doi: 10.1074/jbc.M109.009605

FIGURE 1.

FIGURE 1.

hSUV3 and hPNPase form a 330kDa heteropentameric complex at a 2:3 molar ratio. A, purification scheme of the hSUV3-hPNPase complex. B, size exclusion chromatography (Superdex 200) elution profiles for hSUV3, hPNPase, and the hSUV3-hPNPase complex. The numbers to the right are the approximate molecular masses of the purified proteins based on their peak elution volumes. C, native PAGE (6%, pH 10.0) migration pattern of the purified proteins. D, Coomassie Blue staining of the purified proteins in 8% SDS-PAGE. E and F, Western blotting of the purified proteins in 8% SDS-PAGE. G, UV absorbance curves and the calculated molecular masses of the purified proteins determined by equilibrium sedimentation analytical ultracentrifugation.