FIGURE 4.

C11 is internalized and concentrated in slice cultures. A, untreated homogenate was incubated in triplicate for 24 h at 37 °C with varying (1.0–0.003 μm) concentrations of C11. Fluorescence signal was then plotted against C11 concentration and a linear curve fit to the data (y = 10442x ± 128.17). B, in 0.001 μm treated (T) and control (C) slices (n = 6 mice per group) fluorescence was 183 ± 61.7 and 80.2 ± 33.3, respectively. C, average fluorescence was measured in homogenates (n = 6 per group) of 1 μm C11 treated (T) and control (C) slices. Values obtained were 32,504.8 ± 8,212.9 and 153.8 ± 38.9, respectively. Utilizing the equation of the standard curve, the C11 concentration in 1 μm and 0.001 μm treated samples was estimated as 3.1 and 0.0053 μm, respectively.