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. 2009 May 28;284(31):20830–20839. doi: 10.1074/jbc.M109.016089

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — C11 treatment results in changes in filament mass and number. Filaments in the Sarkosyl-insoluble fraction were negatively stained with uranyl acetate. Image J was used to measure total filament length obtained for each electron micrograph and the average for each condition was calculated. A, total filament number as a percentage of control was determined. A significant increase (p = 0.01) was observed at 1 μm (207.96%), whereas treatment with 0.001 μm produced a significant decrease (p = 0.01) (76.24% of control) B, significantly higher (p < 0.001) total filament mass was observed at 1 μm C11 (317.1% of control). In contrast, 0.001 μm treated samples showed a significant reduction (p < 0.001) to 23.63% of control values. C and D, the number of filaments falling into each 10-nm length range was determined and expressed as a percentage of total filament number. Distributions were similar for control (black bars) and treated (gray bars) samples. E and F, electron micrographs from 1 μm and 0.001 μm treated samples, respectively, are shown.