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. 2009 May 28;284(31):20830–20839. doi: 10.1074/jbc.M109.016089

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — C11 treatment does not negatively affect slice viability. Total protein fractions were assayed for relative levels of cleaved PARP, tubulin, and synaptophysin to ascertain the health of the cultures. A and C, slices incubated with 1 μm C11 showed no difference in cleaved PARP (CP) (92.2 ± 26.5) or synaptophysin (Syn) (90.04 ± 21.1) signal relative to control, however, a marked decrease in tubulin (Tub) immunoreactivity was observed (44.5 ± 27.5). B and D, no changes in the relative concentration of any of the proteins was observed with 0.001 μm C11 (90.9 ± 28.4 for cleaved PARP, 117.3 ± 26.5 for synaptophysin (Syn), and 103.8 ± 28.5 for tubulin (Tub)).