Skip to main content
. 2009 Jun 12;284(31):20927–20935. doi: 10.1074/jbc.M109.022350

FIGURE 1.

FIGURE 1.

HIC1 overexpression in U2OS osteosarcoma cells induces a proliferation arrest. A, expression of HIC1 and GFP in infected U2OS cells. U2OS cells were infected with Ad-FLAG-HIC1 (left panel) or Ad-GFP (right panel), and 24 h later the expression of HIC1 and GFP was analyzed. The HIC1 protein is detected by immunofluorescence with the anti-FLAG M2 monoclonal antibody in a punctate nuclear pattern. B, expression of HIC1 proteins in Ad-FLAG-HIC1-infected U2OS cells. Equal amounts of total protein extracts were subjected to Western blotting (WB) using the anti-FLAG monoclonal antibody. C, overexpression of HIC1 through adenoviral infection induces a growth arrest in U2OS cells. U2OS osteosarcoma cells were seeded in 60-mm plates and infected with Ad-FLAG-HIC1 or Ad-GFP at a multiplicity of infection of 100 to allow infection of at least 90% of the cells. At the indicated times, cell counts were made on five microscopic fields, and each point is representative of the average number of noninfected control cells, Ad-FLAG-HIC1, and Ad-GFP U2OS-infected cells.