FIGURE 2.

CXCR7 is down-regulated in Ad-FLAG-HIC1-infected U2OS cells, whereas SIRT1 is activated. A, expression levels of CXCR7 and of SIRT1. Total RNAs from U2OS cells (HIC1 null) infected with Ad-FLAG-HIC1 and Ad-GFP were prepared at the indicated times after infection (from 8 to 26 h), and Affymetrix HG U133A chips were used to measure the gene expression. Expression values were normalized to Ad-GFP-infected control cells at the same time points. The Percent of control corresponds to the ratio between the expression levels of CXCR7 and SIRT1 measured in Ad-GFP and in Ad-FLAG-HIC1-infected cells at each time point. B, confirmation of the microarray results for CXCR7 and SIRT1 by quantitative RT-PCR. qRT-PCR analyses were performed using total RNAs isolated from U2OS cells infected (time course point 16 h) with Ad-GFP (gray boxes) or with Ad-FLAG-HIC1 (black boxes) for CXCR7 (left column) and SIRT1 (right column). Values were normalized to GAPDH or actin as indicated.