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. 2009 Jun 11;284(31):20946–20955. doi: 10.1074/jbc.M109.023721

FIGURE 2.

FIGURE 2.

Increased luciferase activity by AUF1 silencing. A, HEK293 stably transfected cells lipofected with siAUF1 for 72 h and half-doses for a further 72 h were Western blot analyzed to test the levels of AUF1 isoforms. Upper panel, the gel refers to B4-bARE cells, similar findings were obtained from all siAUF1-treated clones in three independent experiments. Lower panel, densitometric quantifications of AUF1 Western blot bands, normalized to housekeeping loading control protein, are shown as percentage of unsilenced cells. The data shown are the mean of three independent experiments ± S.E. (error bars). B, Renilla luciferase activity was measured and data normalized to protein content. Values are expressed as relative luciferase activity versus cells treated with lipofectamine only. Data are the mean of three independent experiments ± S.E. (error bars). *, p < 0.05; **, p < 0.01 compared with vehicle-treated cells.