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Two-dimensional TLC patterns of M. marinum polar glycolipids. Following extraction from MmaM (A) or Mma7 (B), polar glycolipids were separated on two-dimensional TLC using the solvent system chloroform/methanol/water (60:30:6, v/v) in the first direction and chloroform/acetic acid/methanol/water (40:25:3:6, v/v) in the second direction. Glycolipids were detected with orcinol/sulfuric acid stain.
