FIGURE 3.
Impaired lateral branching in Klf6-deficient anterior prostates. Microdissected prostate from Klff/fNkx3.1+/+ (A and C) and Klff/fNkx3.1Cre/+ (B and D) mice were imaged using ex vivo micro-CT and three-dimensional images were reconstructed. Ductal tips (A and B) and branch points (C and D) were marked on the three-dimensional images, counted (n = 4 from each genotype) and plotted in bar graphs, respectively (E and F). The number of ductal tips and branch points sprouting out of the main ducts are significantly more in (A, C, and E) Klff/fNkx3.1+/+ than (B, D, and F) Klff/fNkx3.1Cre/+ prostates. Statistical significance was calculated using Student's t test, and the bars represent mean ± S.E. Bar, 1 mm for A–D.