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Soluble nematode L3 larval extract from T. colubriformis was separated on 11% SDS-PAGE and probed with the specified scFvs or PAB-1 (anti-CarLA mAb). The indicated scFvs were incubated with the blots and bound scFv detected using an anti-E-tag/HRP conjugated mAb. scFv members recognizing the three epitope groups, E1, E2 and E3, are indicated at the top.
