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. 2009 Sep 25;5(9):e1000597. doi: 10.1371/journal.ppat.1000597

Figure 2. Blocking ELISA for epitope analysis of the anti-T. colubriformis CarLA scFvs.

Figure 2

Phage displaying scFv (top row) was added to wells coated with T. colubriformis extract that had been pre-treated with soluble expressed scFv (first column). Blocking by the soluble scFvs is reflected by substantially reduced signals produced by the phage-displayed scFvs. Values represent percent binding compared to PBS controls. Wells displaying greater than 70% signal reduction (blocking) are boxed. The assay was performed in duplicate on at least three separate occasions.