Figure 3. Species specificity of anti-CarLA scFvs representing three different epitope classes, E1, E2 and E3.

Western blots were performed containing hot water extracts of L3 stage larvae prepared from six different species of nematode. Species used were Trichostrongylus colubriformis (T. co), Haemonchus contortus (H. co), Cooperia curticei (C. cu), Caenorhabditis elegans (C. el), Nematodirus spatiger (N. sp) and Teladorsagia circumcincta (T. ci). The indicated scFvs were incubated with the blots and bound scFv was detected using an anti-E-tag/HRP conjugated mAb. Markers (M) indicate the molecular weight mobility shown on left.