Figure 4. Immunofluorescence of living T. colubriformis L3 larvae probed with selected anti-CarLA scFvs individually or in combination.

Live T. colubriformis exsheathed L3 larvae were probed with Tc.C1 scFv alone, Tc.C2 scFv alone, Tc.C3 scFv alone, or all three in combination as indicated. Bound scFv was stained with anti-E-tag/FITC conjugated mAb and detected by fluorescence microscopy.