Figure 1. Identification of potential EV71 3C^pro substrates by 2D gels.

(A) A [³⁵S]-labeled peptide which contains part of EV71 viral polyprotein was treated with wild-type 3C^pro(WT) or mutant 3C^pro (C147S). The uncleaved peptide precursor and 3C^pro cleavage product was indicated. (B) Full scale of 2D gels for one of the six in vitro cleavage experiments; left panel shows results obtained using the mutant 3C^pro-treated nuclear extract (3C_mut); right panel shows the results obtained using wild-type 3C^pro-treated nuclear extract (3C_WT). Arrows indicate location of CstF-64. (C) Six in vitro cleavage experiments on 2D gels. Arrows indicate location of CstF-64 protein on mutant 3C^pro-treated nuclear extracts gels; circles indicate the corresponding regions on wild-type 3C^pro-treated nuclear extract gels.