Skip to main content
. Author manuscript; available in PMC: 2009 Sep 14.
Published in final edited form as: Biochemistry. 2005 Dec 20;44(50):16574–16583. doi: 10.1021/bi0518186

Figure 5. Double-reciprocal plots of the global fit of the initial velocities of the AaLpxC initiated reactions at increasing concentrations of CHIR-090.

Figure 5

These data were collected in an independent experiment in which the concentration of substrate was also varied. The Ki value for CHIR-090 determined from this experiment was 1.00 ± 0.15 nM, and the KM for UDP-3-O-acyl-N-acetylglucosamine was 1.6 ± 0.2 μM, using Equation 2. The values calculated are the averages from to two separately fit sets of data.