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. Author manuscript; available in PMC: 2009 Sep 14.
Published in final edited form as: Biochemistry. 2005 Dec 20;44(50):16574–16583. doi: 10.1021/bi0518186

Figure 6. LC/ESI-MS analysis of AaLpxC pre-incubated with CHIR-090.

Figure 6

AaLpxC (12.6 μM) and CHIR-090 (5 μM) were incubated for two h at 30 °C and then subjected to LC/ESI-MS analysis. Panel A) Total ion chromatogram of the LC. Panel B) Positive ion mass spectrum collected at 9.9 min. The ion at m/z 438.2 matches the expected [M + H]+ for unmodified CHIR-090. Panel C) Positive ion mass spectrum collected at 14.95 min, showing the multiple charge states of the LpxC protein. Panel D) Deconvoluted spectrum from panel C. The calculated average neutral mass (32,015) is within experimental error of the molecular weight of AaLpxC, lacking a methionine residue (32,013) (41). There was no evidence for a CHIR-090 adduct with AaLpxC.