Table II.
Genomic instability in proliferating B cellsa
| experiment | genotype | metaphases analyzed | # of aneuploid metaphases (%) | # of metaphases with chromosomal aberrations (%) | # of chromosomal aberrations (% per chromosome) |
|---|---|---|---|---|---|
| 1 | Blmf/Δ Cre | 48 | 17 (35%) | 30 (63%) | 84 (4.13%) |
| Blmf/Δ | 47 | 9 (19%) | 2 (4%) | 2 (0.10%) | |
| 2 | Blmf/Δ Cre | 42 | 19 (45%) | 16 (38%) | 27 (1.52%) |
| Blmf/+ | 52 | 9 (17%) | 5 (10%) | 5 (0.23%) | |
| 3 | Blmf/Δ Cre | 50 | 18 (36%) | 27 (54%) | 50 (2.42%) |
| Blmf/+ Cre | 58 | 11 (19%) | 8 (14%) | 17 (0.72%) |
a
Chromosome breaks, translocations, small marker chromosomes, double minutes, and prematurely condensed chromosomes were scored on Giemsa-stained metaphase spreads prepared from CD43− B cells that had been stimulated with anti-CD40 and IL4 for three days. Cells from one mouse per genotype were used for each experiment.