FIGURE 2.

Localization of sequences downstream from insertion sites required for editing. (A,B) Template alterations downstream from editing site 9 (es9) within the gene encoding the mitochondrial small ribosomal rRNA (SSU). Circle template CD1 was created by fusion at PstI sites within the SSU and LSU (large rRNA) genes. Hybrid cassette template HC1 has exogenous DNA ligated at the same SSU site. Junction regions are expanded to indicate substituted nucleotides, shown in uppercase. Shaded regions indicate downstream sequence identical to that of native mtTECs. Editing sites within each RT-PCR product are indicated. The editing status of individual editing sites is shown schematically, with filled diamonds representing correctly edited sites, empty diamonds indicating unedited sites, and mis-edited sites as indicated in the legend. Both CD1 and HC1 support C insertion at SSU es9. Only independent clones (i.e., clones having unique editing patterns) are shown for each construct. (C, top) Junction regions of other templates with alterations downstream from editing sites. The ability of each template to support accurate editing at the site nearest the junction is indicated at the right. (Bottom) The editing status of individual sites in independent CD4 cDNA clones is shown as in A and B.