FIGURE 2.

Depletion of decoding region modifications can impair cell growth and increase cell sensitivity to neomycin. (A) Growth comparisons for strains depleted of P-region Ψ (−1a), Aa-region Ψ (−1b), and Aa-region Nm (−1c) individually or in different combinations. Two OD₆₀₀ units of cells were diluted serially (1:5), spotted on plates with rich YPD medium in the presence (right panel) or absence (left panel) of neomycin (0.4 mg/mL), and incubated at different temperatures. (B) Growth properties of strains depleted of Aa-region Nm (−1c) and P-region Nm (−1d) individually or in different combinations, as in A. Strain (−)1e is depleted of an A-region Nm (Gm1271). Strains showing cold-sensitive phenotypes (boxed) and increased neomycin sensitivity (arrowheads) are identified. The schematic structures at the left of the data panels indicate the modification situations, as shown in Figure 1B.