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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Dev Dyn. 2009 Mar;238(3):514–523. doi: 10.1002/dvdy.21856

Fig. 2.

Fig. 2

c-fos gene deletion rescues the endoderm disorganization in E5.5 dab2 (−/−) embryos. E5.5 embryos of various genotypes were stained with Dab2 or GATA-4 to determine protein expression (for dab2 genotyping) and organization of the visceral endoderm cells. (A) An example of a wildtype embryo: the visceral endoderm cells are positive for GATA-4 and Dab2, and are organized into an epithelium covering the embryos. (B) An example of dab2 (−/−) embryo from a timed-mating between dab2 (+/−) mice. Lack of Dab2 immunostaining indicates it is a dab2 (−/−) embryo. Note that the GATA-4-positive endoderm cells are disorganized and fail to form a surface endoderm layer. (C) An example of a presumed dab2 (−/−):c-fos (−/−) embryo. Dab2 staining is negative indicating a dab2 (−/−) genotype, while GATA-4-positive visceral endoderm cells appear to organize into an epithelium.