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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cancer Lett. 2009 May 15;284(2):175–181. doi: 10.1016/j.canlet.2009.04.022

Figure 4.

Figure 4

4HPR inhibits MM-induced tube formation by HUVECs. A-C) HUVEC (15,000 cells/well) were cultured on Matrigel in 96-well plates for 3 hrs in the presence or absence of indicated concentrations of 4HPR. Tube formations were tested in plain medium (P. Med), HUVEC growth medium (G. Med), ARP-I conditioned medium (ARP-1 CM, 50% in plain medium) and CAG cell conditioned medium (CAG CM, 50% in plain medium). Note that 4HPR inhibited tube formation induced by growth factors or MM cell conditioned media in a dose-depended manner. D) HUVEC were cultured in 96-well plate (10,000 cells per well) and treated with indicated concentrations of 4HPR for 4 hours. Following treatment HUVEC cells were washed with PBS and stained with Annexin V according to the manufacturer’s instructions (see Materials and Methods). Note that under the experimental conditions 4HPR induced apoptosis at concentrations higher than 6 μM.