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Adult olfactory bulb slice before (A) and after (B) layer microdissection. A: Each layer in the 300-μm-thick vibratome slices through the olfactory bulb is clearly visible. B: After microdissection, the different olfactory bulb layers are collected and processed for RNA extraction. ONL, olfactory nerve fiber layer; GL, glomerular layer; EPL, external plexiform layer; MCL, mitral cell layer; GCL, granule cell layer; SEL, subependymal layer. Scale bar = 1 mm in A; 200 μm in B.
