. Author manuscript; available in PMC: 2010 Apr 1.

Published in final edited form as: Bioconjug Chem. 2009 Apr;20(4):808–816. doi: 10.1021/bc800313c

Table 2.

Bromination of 4 Using Different Oxidizing Agents^a

#	4^b (µmol)	NH₄Br^c (µmol)	Oxidizer (µmol)	Reaction Time (min)	Temperature (°C)	Relative Yield^d 2 (%)
1	0.17	0.06	PAA (39.4) AcOH (100 µL)	15	RT	(100)
2	0.17	0.06	CAT (0.44) MeOH (100 µL)	15	RT	0.3
3	0.17	0.06	2:1 H₂O₂/AcOH (1960) (100 µL)	15	RT	0
4	0.17	0.06	CAT (0.44) MeOH/AcOH (100 µL/100 µL)	15 40	RT RT	40 60
5	0.17	0.06	CAT (0.44) MeOH/AcOH (100 µL/100 µL)	10 20	88 88	89 90

Data from single experiment

4 (100 µg, 0.17 µmol) in MeOH (100 µL)

a solution of NH₄Br (6 µg, 0.06 µmol) in H₂O (10 µL)

Yields are given relative to that obtained in reaction #1, based on HPLC integrations of 2.