. Author manuscript; available in PMC: 2010 Jun 30.

Published in final edited form as: J Immunol Methods. 2009 Apr 15;345(1-2):49–59. doi: 10.1016/j.jim.2009.04.002

Table 1.

Ki (equilibrium dissociation constant in a competition assay) of PE and aGalCer for CD1 molecules. Ki was calculated based on the data in Figure 3C, D and E. Kds of B-PE (CD1b: 2.69 (1.11–4.26), CD1c: 2.28 (0.76–3.79), CD1d: 1.69 (0.64–2.87)) was determined by titrating the amount B-PE in the absence of competitor lipids (data not shown) and used to calculate Kis of the competitor lipids by the equation (Ki=IC₅₀/(1+[B-PE]/Kd)). The figures in the bracket represent 95% confidential interval (95%CI).

	CD1b Ki (μM)	CD1c Ki (μM)	CD1d Ki (μM)
PE	1.19 (0.77–1.84)	2.26 (1.15–4.45)	1.46 (0.86–2.47)
α-GalCer	2.84 (1.72–4.69)	1.37 (0.90–2.08)	0.097 (0.07–0.14)