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Osteopontin receptors are expressed on human cancer cells. Cultured human C8161 melanoma and KRIB osteosarcoma cells were immunostained for two of the major osteopontin receptors: αv integrins and CD44. Abundant punctuate αv integrin immunoreactivity was detected in all cell types. The same cells displayed also CD44 immunoreactivity. Negative staining controls with irrelevant isotype control plus secondary antibodies displayed no immunoreactivity. Scale bar, 20 μm.
