Skip to main content
. 2009 Sep 23;4(9):e7097. doi: 10.1371/journal.pone.0007097

Figure 5. Twinfilin-2 interacts with myosinVIIa in vivo.

Figure 5

The interaction between twinfilin-2 and myosinVIIa was demonstrated by immunoprecipitation from protein lysates of inner ear tissue with anti myosinVIIa antibody (myoVIIa), anti twinfilin-2 antibody (twf-2), protein A-Sepharose and anti twinfilin-1 (twf-1) antibody followed by the immunoblot with a anti-myosinVIIa antibody (A) and anti-twinfilin-2 antibody (B). Procedures for immunoprecipitations and Western blots are described under Materials and Methods (Supp. information). Twinfilin-2 has a molecular weight of 39 kD. MyosinVIIa has a molecular weight of about 250 kD; the band at 100 kD may be a result of protein degradation.