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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Crit Care Med. 2009 Jun;37(6):2000–2009. doi: 10.1097/CCM.0b013e3181a001ae

Figure 2. Verification of Recombinant Human TFAM and HMGB1 Proteins.

Figure 2

(A) Stained protein gel of TFAM and HMGB1 (3μg). TFAM.myc.6×His and HMGB1.myc.6×His monomers were ∼29 kD. (B) Western blot of proteins (2 μg) using antibodies directed against TFAM and HMGB1. (C-D) Protein gels of TFAM and HMGB1 (2 μg) following incubation with annealed oligonucleotide binding probes [32P-LSP (C) or 32P-cruciform (D) DNA].