. Author manuscript; available in PMC: 2010 Jun 1.

Published in final edited form as: Crit Care Med. 2009 Jun;37(6):2000–2009. doi: 10.1097/CCM.0b013e3181a001ae

Table 2.

Mitochondrial DNA (mtDNA)-Encoded Gene Expression by Real-Time PCR.

Gene	Normal HepG2 Cell [(EtBr-)HepG2]	Ethidium Bromide-Treated HepG2 Cell [(EtBr+)HepG2]
Gene	Relative Copy Number	Relative Copy Number	Fold Difference (Reduced)
ATP synthase F0 6	66	0.023	2815
Cytochrome c oxidase I	181	0.047	3807
Cytochrome c oxidase II	99	0.024	4141
Cytochrome c oxidase III	73	0.015	5041
Cytochrome c oxidase IV^a	1	0.046	20
Cytochrome b	49	0.011	4326
NADH dehydrogenase 1	29	0.047	611
NADH dehydrogenase 2	64	0.036	1753
NADH dehydrogenase 3	26	0.006	4472
NADH dehydrogenase 4	114	0.024	4813
NADH dehydrogenase 4L	94	0.025	3843
NADH dehydrogenase 5	98	0.027	3593
NADH dehydrogenase 6	58	0.012	4944

The expression of cytochrome c oxidase IV, a nuclear gene product, in normal HepG2 cells was used to normalize the expression of all other genes.