Fig. 3.

WNK2 is frequently affected by aberrant methylation in grade II and III meningiomas. (A) Schematic drawing showing the WNK2-associated CpG island and the location of five fragments for bisulfite sequencing analysis (“seq. assay”). The methylation status of individual CpG sites within this CpG island was calculated from clones of bisulfite sequencing analysis. An average of 11 clones (range, 7–20) were sequenced for each fragment. The numbers by each fragment represent the position of the fragment relative to the translation start site (ATG). (B–D) The methylation rate of these fragments in nontumor leptomeninges (NB1, NB2; B), grade II (m24, m25) and grade III (m30, m33) meningiomas (C), and cell lines (IOMM-Lee, KT21-MG1; D). CpG sites 1–22 were analyzed from fragment I; sites 23–97, from fragment II; sites 98–104, from fragment III; sites 105–120, from fragment IV; and sites 122–156, from fragment V.