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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: J Med Chem. 2009 Jul 9;52(13):3829–3845. doi: 10.1021/jm801420y

Table 2.

Assay methods used in each discovery program

Assay method Compounds
Chick embryo neural plate explant 3-10 95
Shh-responsive luciferase reporter assay in a colonal cell line derived from NIH-3T3 11-16 96
Alkaline phostphatase 1-2,85 17-24,100 27-34,104 35-45105
Shh-LIGHT-II 1-2,85 50-53,109 72-76,113 115-120,131 125-127124
Gli-responsive luciferase reporter (no exogenous Gli) 54-66,110, 111 67-71,112 78-86,115 87,117, 88-91,118 92-96,119 101-103,125 104-106,126 107-114,129 123-124,134 128-129,140
Smo binding 78-86115
Gli-responsive luciferase reporter with exogenous Gli1 or Gli2 130-139,141, 142 140-145,143 146-151