Table 3.
List of the interactive sequences in human αB crystallin involved in chaperone activity and filament interactions. Column 1 lists the structural domain in human αB crystallin where each interactive sequence is located. Column 2 lists the chaperone sequences identified previously (Ghosh, Estrada, & Clark, 2005). Columns 3-5 list the sequences in human αB crystallin for interactions with desmin, GFAP, and actin respectively. Sequences that interacted with actin at 23°C are in BOLD. Sequences that interacted with partially unfolded actin at 45°C are in regular font. Common αB crystallin sequences interacted with filaments and unfolded cytosolic proteins (Ghosh, Estrada, & Clark, 2005).
Location | Interactive sequences | Desmin sequences | GFAP sequences | Actin sequences |
---|---|---|---|---|
N-terminus | 9WIRRPFFPFHSP20 | 15FPFHSPSRLF24 | 9WIRRPFFP16 | 15FPFHSPSRLFDQ26 |
N-terminus | 43SLSPFYLRPPSFLRAP58 | 41STSLSPFYLRPPSFLRAPSWFD62 | 45SPFYLRPPSFLRAPSWFD62 | 33LESDLFPTSTSLSPFYLRPPSFLR56 |
N-terminus | - | - | - | 57APSWFDTG64 |
α crystallin core domain | 75FSVNLDVK82 | 75FSVNLDVKHFSPEELK90 | 89LKVKVLGDVI98 | - |
α crystallin core domain | 113FISREFHR120 | 113FISREFHRKYRIPADV128 | 113FISREFHRKYRIPADV128 | 111HGFISREF118 |
α crystallin core domain | 131LTITSSLS138 | 131LTITSSLS138 | - | 129DPLTITSSLSSDGV145 |
α crystallin core domain | 141GVLTVNGP148 | 145VNGPRKQV152 | 143LTVNGPRK150 | 145VNGPRKQVSG154 |
C-terminus | 157ERTIPITRE164 | 163REEKPAVTAAPK174 | 155PERTIPITREEKPAVTAA172 | 155PERTIPITREEK165 |