Relative gene expression of (A) type I collagen, (B) type II collagen, and (C) aggrecan for all HA hydrogel formulations after 3 (white) and 14 (black) days of culture. Each sample was internally normalized to GAPDH, and each group was normalized to expression of MSCs isolated at the time of encapsulation. Statistical analysis of relevant comparisons: * denotes significant difference between starred groups and all other groups for the specified time point and # denotes significant difference between bracketed groups. Relative gene expression of type II collagen for 5, 2, and 1 wt% MeHA only hydrogels were also all significantly different from each other at day 14. Values are reported as mean ± standard deviation.