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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mech Ageing Dev. 2009 Mar 24;130(6):384–392. doi: 10.1016/j.mad.2009.03.002

Figure 4. DX5+ bone marrow cells, but not dendritic cells, inhibit E47 expression and increase pERK expression in B cell precursors.

Figure 4

A and B, B cell precursors (CD19+) from young BALB/c mice were sorted by flow cytometry and co-cultured with the following sorted bone marrow cell subsets from young mice and placed in transwells: total DC (CD19- CD11c+); pDCs (CD19- B220+ CD11c+); or NK cells (DX5+) at 1:5 ratios. Cultures also contained 5ng/ml IL-7 and were harvested at 7 days and Western blots performed for E47, pERK and the loading control Ubc9. Cumulative results for E47 protein levels for 3 experiments are shown in C and for phospho-ERK protein in D normalized to loading controls. * p≤0.05 by Student's t-test for CD19+ vs. CD19+ cells + NK, tDC, or pDC groups. Data for E47 protein was normalized to loading control values [E47/Ubc9 ratio or pErk/Ubc9 ratio] in Western blots.